Article
Directed evolution of a synthetic phylogeny of programmable Trp repressors.
Nature chemical biology - 1 Apr 2018
Ellefson Jared W, Ledbetter Michael P, Ellington Andrew D
Abstract excerpt
As synthetic regulatory programs expand in sophistication, an ever increasing number of biological components with predictable phenotypes is required. Regulators are often 'part mined' from a diverse, but uncharacterized, array of genomic sequences, often leading to idiosyncratic behavior. Here, we generate an entire synthetic phylogeny from the canonical allosteric transcription factor TrpR. Iterative rounds of...
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