Article
Directed evolution of the tryptophan synthase β-subunit for stand-alone function recapitulates allosteric activation.
Proceedings of the National Academy of Sciences of the United States of America - 24 Nov 2015
Buller Andrew R, Brinkmann-Chen Sabine, Romney David K, Herger Michael, Murciano-Calles Javier, Arnold Frances H
Abstract excerpt
Enzymes in heteromeric, allosterically regulated complexes catalyze a rich array of chemical reactions. Separating the subunits of such complexes, however, often severely attenuates their catalytic activities, because they can no longer be activated by their protein partners. We used directed evolution to explore allosteric regulation as a source of latent catalytic potential using the β-subunit of tryptophan...
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