Article
Intact Cell Assays to Monitor AMPK and Determine the Contribution of the AMP-Binding or ADaM Sites to Activation.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018
Hawley Simon A, Fyffe Fiona A, Russell Fiona M, Gowans Graeme J, Grahame Hardie D
Abstract excerpt
AMP-activated protein kinase (AMPK) is extremely sensitive to cellular stress, so that nonphysiological activation of the kinase can readily occur during harvesting of cells or tissues. In this chapter we describe methods to harvest cells and tissues, and for kinase assays, that preserve the physiological activation status of AMPK as far as possible. Note that similar care with methods of cell or tissue...
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