Article
Inducible CRISPR genome editing platform in naive human embryonic stem cells reveals JARID2 function in self-renewal.
Cell cycle (Georgetown, Tex.) - 1 Jan 2018
Ferreccio Amy, Mathieu Julie, Detraux Damien, Somasundaram Logeshwaran, Cavanaugh Christopher, Sopher Bryce, Fischer Karin, Bello Thomas, M Hussein Abdiasis, Levy Shiri, Cook Savannah, Sidhu Sonia B, Artoni Filippo, Palpant Nathan J, Reinecke Hans, Wang Yuliang, Paddison Patrick, Murry Charles, Jayadev Suman, Ware Carol, Ruohola-Baker Hannele
Abstract excerpt
To easily edit the genome of naïve human embryonic stem cells (hESC), we introduced a dual cassette encoding an inducible Cas9 into the AAVS1 site of naïve hESC (iCas9). The iCas9 line retained karyotypic stability, expression of pluripotency markers, differentiation potential, and stability in 5iLA and EPS pluripotency conditions. The iCas9 line induced efficient homology-directed repair (HDR) and non-homologous...
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