Article
Genotypic detection of fluoroquinolone resistance in drug-resistant Mycobacterium tuberculosis at a tertiary care centre in south Coastal Karnataka, India.
Journal of global antimicrobial resistance - 1 Jun 2018
Chawla Kiran, Kumar Ajay, Shenoy Vishnu Prasad, Chakrabarty Sanjiban, Satyamoorthy Kapaettu
Abstract excerpt
OBJECTIVES: This study aimed to characterise mutations associated with fluoroquinolone resistance in drug-resistant Mycobacterium tuberculosis clinical isolates at a tertiary care centre in south Coastal Karnataka, India. METHODS: DNA from 50 M. tuberculosis clinical isolates was extracted and the gyrA and gyrB genes were amplified. Purified amplicons of gyrA and gyrB were sequenced and extended-sequencing PCR...
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