Article
Sequence-specific DNA binding activity of the cross-brace zinc finger motif of the piggyBac transposase.
Nucleic acids research - 16 Mar 2018
Morellet Nelly, Li Xianghong, Wieninger Silke A, Taylor Jennifer L, Bischerour Julien, Moriau Séverine, Lescop Ewen, Bardiaux Benjamin, Mathy Nathalie, Assrir Nadine, Bétermier Mireille, Nilges Michael, Hickman Alison B, Dyda Fred, Craig Nancy L, Guittet Eric
Abstract excerpt
The piggyBac transposase (PB) is distinguished by its activity and utility in genome engineering, especially in humans where it has highly promising therapeutic potential. Little is known, however, about the structure-function relationships of the different domains of PB. Here, we demonstrate in vitro and in vivo that its C-terminal Cysteine-Rich Domain (CRD) is essential for DNA breakage, joining and...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.