Article
Modulation of pPS10 host range by DnaA.
Molecular microbiology - 1 Oct 2002
Maestro Beatriz, Sanz Jesús M, Faelen Michel, Couturier Martine, Díaz-Orejas Ramón, Fernández-Tresguerres Elena
Abstract excerpt
Narrow-host-range plasmid pPS10, originally found in Pseudomonas savastanoi, is unable to replicate in other strains such as Escherichia coli. Here, we report that the establishment of pPS10 in E. coli can be achieved by a triple mutation in the dnaA gene of E. coli (dnaA403), leading to Q14amber, P297S and A412V changes in the DnaA host replication protein (DnaA403 mutant). As the E. coli strain used contained...
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