Article
Identification and characterization of novel ColE1-type, high-copy number plasmid mutants in Legionella pneumophila.
Plasmid - 1 Nov 2006
Chen Ding-qiang, Zheng Xiao-cong, Lu Yong-jun
Abstract excerpt
ColE1-type plasmids are commonly used in bacterial genetics research, and replication of these plasmids is regulated by interaction of RNA I and RNA II. Although these plasmids are narrow-host-range, they can be maintained in Legionella pneumophila under antibiotic selection, with low-copy number and instability. Here, we have described the isolation of two novel spontaneous mutants of pBC(gfp)Pmip, pBG307 and...
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