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Article

Conditional Manipulation of Gene Function in Human Cells with Optimized Inducible shRNA.

Current protocols in stem cell biology - 28 Feb 2018

Bertero Alessandro, Yiangou Loukia, Brown Stephanie, Ortmann Daniel, Pawlowski Matthias, Vallier Ludovic

Abstract excerpt

The difficulties involved in conditionally perturbing complex gene expression networks represent major challenges toward defining the mechanisms controlling human development, physiology, and disease. We developed an OPTimized inducible KnockDown (OPTiKD) platform that addresses the limitations of previous approaches by allowing streamlined, tightly-controlled, and potent loss-of-function experiments for both...

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PMID: 29512130
DOI: 10.1002/cpsc.45

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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