Article
Comparison of Various Nuclear Localization Signal-Fused Cas9 Proteins and Cas9 mRNA for Genome Editing in Zebrafish.
G3 (Bethesda, Md.) - 2 Mar 2018
Hu Peinan, Zhao Xueying, Zhang Qinghua, Li Weiming, Zu Yao
Abstract excerpt
The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has been proven to be an efficient and precise genome editing technology in various organisms. However, the gene editing efficiencies of Cas9 proteins with a nuclear localization signal (NLS) fused to different termini and Cas9 mRNA have not been systematically compared. Here, we compared the ability of Cas9 proteins with NLS fused...
Topics
- Animals
- CRISPR-Cas Systems
- Clustered Regularly Interspaced Short Palindromic Repeats
- Endonucleases
- Gene Editing
- Gene Knockout Techniques
- Gene Targeting
- Genome
- Microinjections
- Mutagenesis
- Mutation