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Bacterial Genome Editing Strategy for Control of Transcription and Protein Stability.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018

Lauritsen Ida, Martínez Virginia, Ronda Carlotta, Nielsen Alex Toftgaard, Nørholm Morten H H

Abstract excerpt

In molecular biology and cell factory engineering, tools that enable control of protein production and stability are highly important. Here, we describe protocols for tagging genes in Escherichia coli allowing for inducible degradation and transcriptional control of any soluble protein of interest. The underlying molecular biology is based on the two cross-kingdom tools CRISPRi and the N-end rule for protein...

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PMID: 29170951
DOI: 10.1007/978-1-4939-7295-1_3

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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