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Article

Fusion guide RNAs for orthogonal gene manipulation with Cas9 and Cpf1.

Nature communications - 23 Nov 2017

Kweon Jiyeon, Jang An-Hee, Kim Da-Eun, Yang Jin Wook, Yoon Mijung, Rim Shin Ha, Kim Jin-Soo, Kim Yongsub

Abstract excerpt

The bacteria-derived clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems are powerful tools for genome engineering. Recently, in addition to Cas protein engineering, the improvement of guide RNAs are also performed, contributing to broadening the research area of CRISPR-Cas9 systems. Here we develop a fusion guide RNA (fgRNA) that functions with both Cas9 and Cpf1 proteins to induce...

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Share to Artificial Gene Fusion
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 29167440
DOI: 10.1038/s41467-017-01650-w
PMCID: PMC5700056

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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