Article

Comprehensive single-cell genome analysis at nucleotide resolution using the PTA Analysis Toolbox

2023-02-15

Abstract excerpt

Detection of somatic mutations in single cells has been severely hampered by technical limitations of whole genome amplification. Novel technologies including primary template-directed amplification (PTA) significantly improved the accuracy of single-cell whole genome sequencing (WGS), but still generate hundreds of artefacts per amplification reaction. We developed a comprehensive bioinformatic workflow, called t...

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Literature Corpus work: edd9388d-f7bb-5588-8189-785f19f283b8
DOI: 10.1101/2023.02.15.528636

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