Article
Post-conversion targeted capture of modified cytosines in mammalian and plant genomes.
Nucleic acids research - 13 Jul 2015
Li Qing, Suzuki Masako, Wendt Jennifer, Patterson Nicole, Eichten Steven R, Hermanson Peter J, Green Dawn, Jeddeloh Jeffrey, Richmond Todd, Rosenbaum Heidi, Burgess Daniel, Springer Nathan M, Greally John M
Abstract excerpt
We present a capture-based approach for bisulfite-converted DNA that allows interrogation of pre-defined genomic locations, allowing quantitative and qualitative assessments of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) at CG dinucleotides and in non-CG contexts (CHG, CHH) in mammalian and plant genomes. We show the technique works robustly and reproducibly using as little as 500 ng of starting...
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