Article
Modification of single-nucleotide polymorphism in a fully humanized CYP3A mouse by genome editing technology.
Scientific reports - 9 Nov 2017
Abe Satoshi, Kobayashi Kaoru, Oji Asami, Sakuma Tetsushi, Kazuki Kanako, Takehara Shoko, Nakamura Kazuomi, Okada Azusa, Tsukazaki Yasuko, Senda Naoto, Honma Kazuhisa, Yamamoto Takashi, Ikawa Masahito, Chiba Kan, Oshimura Mitsuo, Kazuki Yasuhiro
Abstract excerpt
Cytochrome P450, family 3, subfamily A (CYP3A) enzymes metabolize approximately 50% of commercially available drugs. Recently, we developed fully humanized transchromosomic (Tc) CYP3A mice with the CYP3A cluster including CYP3A4, CYP3A5, CYP3A7, and CYP3A43. Our humanized CYP3A mice have the CYP3A5*3 (g.6986G) allele, resulting in the almost absence of CYP3A5 protein expression in the liver and intestine. To...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.