Article
Glycosylphosphatidylinositol (GPI) anchored protein deficiency serves as a reliable reporter of Pig-a gene Mutation: Support from an in vitro assay based on L5178Y/Tk+/- cells and the CD90.2 antigen.
Environmental and molecular mutagenesis - 1 Jan 2018
Bemis Jeffrey C, Avlasevich Svetlana L, Labash Carson, McKinzie Page, Revollo Javier, Dobrovolsky Vasily N, Dertinger Stephen D
Abstract excerpt
Lack of cell surface glycosylphosphatidylinositol (GPI)-anchored protein(s) has been used as a reporter of Pig-a gene mutation in several model systems. As an extension of this work, our laboratory initiated development of an in vitro mutation assay based on the flow cytometric assessment of CD90.2 expression on the cell surface of the mouse lymphoma cell line L5178Y/Tk+/- . Cells were exposed to mutagenic and...
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