Article
Quantifying variant differences in DNA melting curves: Effects of length, melting rate, and curve overlay.
Analytical biochemistry - 15 Dec 2017
Li M, Palais R A, Zhou L, Wittwer C T
Abstract excerpt
High resolution DNA melting of PCR products is a simple technique for sequence variant detection and analysis. However, sensitivity and specificity vary and depend on many factors that continue to be defined. We introduce the area between normalized melting curves as a metric to quantify genotype discrimination. The effects of amplicon size (51-547 bp), melting rate (0.01-0.64 °C/s) and analysis method (curve...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.