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Article

Quantitative heteroduplex analysis for single nucleotide polymorphism genotyping.

Analytical biochemistry - 1 Nov 2005

Palais Robert A, Liew Michael A, Wittwer Carl T

Abstract excerpt

High-resolution melting of polymerase chain reaction (PCR) products can detect heterozygous mutations and most homozygous mutations without electrophoretic or chromatographic separations. However, some homozygous single nucleotide polymorphism (SNPs) have melting curves identical to that of the wild-type, as predicted by nearest neighbor thermodynamic models. In these cases, if DNA of a known reference genotype...

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PMID: 16188219
DOI: 10.1016/j.ab.2005.08.010

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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