Article
Arrayed mutant haploid embryonic stem cell libraries facilitate phenotype-driven genetic screens.
Nucleic acids research - 15 Dec 2017
Liu Guang, Wang Xue, Liu Yufang, Zhang Meili, Cai Tao, Shen Zhirong, Jia Yuyan, Huang Yue
Abstract excerpt
Forward genetic screens using mammalian embryonic stem (ES) cells have identified genes required for numerous cellular processes. However, loss-of-function screens are more difficult to conduct in diploid cells because, in most cases, both alleles of a gene must be mutated to exhibit a phenotype. Recently, mammalian haploid ES cell lines were successfully established and applied to several recessive genetic...
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