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Article

A homozygous mutant embryonic stem cell bank applicable for phenotype-driven genetic screening.

Nature methods - 23 Oct 2011

Horie Kyoji, Kokubu Chikara, Yoshida Junko, Akagi Keiko, Isotani Ayako, Oshitani Akiko, Yusa Kosuke, Ikeda Ryuji, Huang Yue, Bradley Allan, Takeda Junji

Abstract excerpt

Genome-wide mutagenesis in mouse embryonic stem cells (ESCs) is a powerful tool, but the diploid nature of the mammalian genome hampers its application for recessive genetic screening. We have previously reported a method to induce homozygous mutant ESCs from heterozygous mutants by tetracycline-dependent transient disruption of the Bloom's syndrome gene. However, we could not purify homozygous mutants from a...

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PMID: 22020066
DOI: 10.1038/nmeth.1739

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