Article
Assessing miR-451 Activity and Its Role in Erythropoiesis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018
Kretov Dmitry A, Shafik Andrew M, Cifuentes Daniel
Abstract excerpt
The ability to microinject small RNAs and mRNAs into zebrafish embryos, of different genetic backgrounds, allows for the precise dissection of microRNA processing pathways at the molecular level, while simultaneously provides insight into their physiologic role. Here, we apply such an approach to determine the impact of Argonaute 2 in the processing of miR-451, a vertebrate-specific microRNA required for terminal...
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