Article
A directed evolution approach to select for novel Adeno-associated virus capsids on an HIV-1 producer T cell line.
Journal of virological methods - 1 Dec 2017
Wooley Dawn P, Sharma Priyanka, Weinstein John R, Kotha Lakshmi Narayan Poornima, Schaffer David V, Excoffon Katherine J D A
Abstract excerpt
A directed evolution approach was used to select for Adeno-associated virus (AAV) capsids that would exhibit more tropism toward an HIV-1 producer T cell line with the long-term goal of developing improved gene transfer vectors. A library of AAV variants was used to infect H9 T cells previously infected or uninfected by HIV-1 followed by AAV amplification with wild-type adenovirus. Six rounds of biological...
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