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Article

CRISPR-Cas9-assisted recombineering in Lactobacillus reuteri.

Nucleic acids research - 1 Jan 2014

Oh Jee-Hwan, van Pijkeren Jan-Peter

Abstract excerpt

Clustered regularly interspaced palindromic repeats (CRISPRs) and the CRISPR-associated (Cas) nuclease protect bacteria and archeae from foreign DNA by site-specific cleavage of incoming DNA. Type-II CRISPR-Cas systems, such as the Streptococcus pyogenes CRISPR-Cas9 system, can be adapted such that Cas9 can be guided to a user-defined site in the chromosome to introduce double-stranded breaks. Here we have...

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Identifiers

PMID: 25074379
DOI: 10.1093/nar/gku623
PMCID: PMC4176153

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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