Article
Structure/cleavage-based insights into helical perturbations at bulge sites within T. thermophilus Argonaute silencing complexes.
Nucleic acids research - 6 Sept 2017
Sheng Gang, Gogakos Tasos, Wang Jiuyu, Zhao Hongtu, Serganov Artem, Juranek Stefan, Tuschl Thomas, Patel Dinshaw J, Wang Yanli
Abstract excerpt
We have undertaken a systematic structural study of Thermus thermophilus Argonaute (TtAgo) ternary complexes containing single-base bulges positioned either within the seed segment of the guide or target strands and at the cleavage site. Our studies establish that single-base bulges 7T8, 5A6 and 4A5 on the guide strand are stacked-into the duplex, with conformational changes localized to the bulge site, thereby...
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