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Article

Single-Nucleotide Polymorphism Leading to False Allelic Fraction by Droplet Digital PCR.

Clinical chemistry - 1 Aug 2017

Christenson Eric S, Dalton W Brian, Chu David, Waters Ian, Cravero Karen, Zabransky Daniel J, DeZern Amy E, Park Ben Ho

Abstract excerpt

BACKGROUND: Molecular-based diagnostics have great utility for cancer detection. We have used droplet digital PCR (ddPCR) as a platform for identifying mutations in circulating plasma tumor DNA (ptDNA). We present the unexpected finding of a spurious mutant allele fraction that was discovered to be artifactual because of the presence of a single-nucleotide polymorphism (SNP) in a patient sample. DESIGN AND...

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Share to Alleles
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 28615231
DOI: 10.1373/clinchem.2017.273177

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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