Article
Analysis of Base-Position Error Rate of Next-Generation Sequencing to Detect Tumor Mutations in Circulating DNA.
Clinical chemistry - 1 Nov 2016
Pécuchet Nicolas, Rozenholc Yves, Zonta Eleonora, Pietrasz Daniel, Didelot Audrey, Combe Pierre, Gibault Laure, Bachet Jean-Baptiste, Taly Valérie, Fabre Elizabeth, Blons Hélène, Laurent-Puig Pierre
Abstract excerpt
BACKGROUND: Detecting single-nucleotide variations and insertions/deletions in circulating tumor DNA is challenging because of their low allele frequency. The clinical use of circulating tumor DNA to characterize tumor genetic alterations requires new methods based on next-generation sequencing. METHODS: We developed a method based on quantification of error rate of each base position [position error rate (PER)]....
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