Article
Single-mutation fitness landscapes for an enzyme on multiple substrates reveal specificity is globally encoded.
Nature communications - 6 Jun 2017
Wrenbeck Emily E, Azouz Laura R, Whitehead Timothy A
Abstract excerpt
Our lack of total understanding of the intricacies of how enzymes behave has constrained our ability to robustly engineer substrate specificity. Furthermore, the mechanisms of natural evolution leading to improved or novel substrate specificities are not wholly defined. Here we generate near-comprehensive single-mutation fitness landscapes comprising >96.3% of all possible single nonsynonymous mutations for...
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