Decoding the substrate specificity landscape of a promiscuous enzyme through multi-substrate mutational scanning.

Abstract excerpt

Substrate specificity is a defining feature of enzyme function, but its molecular underpinnings remain difficult to decode and engineer. Here, we leverage enzyme proximity sequencing (EP-Seq) to systematically map how single-point and combinatorial mutations reshape the substrate preferences of D-amino acid oxidase (DAOx) from Rhodotorula gracilis, a model promiscuous enzyme. We generate ~40,000...

Topics

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• Catalytic Domain
• Models, Molecular
• Mutation
• Substrate Specificity