Article
Altered expression of a quality control protease in E. coli reshapes the in vivo mutational landscape of a model enzyme.
eLife - 23 Jul 2020
Thompson Samuel, Zhang Yang, Ingle Christine, Reynolds Kimberly A, Kortemme Tanja
Abstract excerpt
Protein mutational landscapes are shaped by the cellular environment, but key factors and their quantitative effects are often unknown. Here we show that Lon, a quality control protease naturally absent in common E. coli expression strains, drastically reshapes the mutational landscape of the metabolic enzyme dihydrofolate reductase (DHFR). Selection under conditions that resolve highly active mutants reveals...
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