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Article

Revealing enzyme functional architecture via high-throughput microfluidic enzyme kinetics.

Science (New York, N.Y.) - 23 Jul 2021

Markin C J, Mokhtari D A, Sunden F, Appel M J, Akiva E, Longwell S A, Sabatti C, Herschlag D, Fordyce P M

Abstract excerpt

Systematic and extensive investigation of enzymes is needed to understand their extraordinary efficiency and meet current challenges in medicine and engineering. We present HT-MEK (High-Throughput Microfluidic Enzyme Kinetics), a microfluidic platform for high-throughput expression, purification, and characterization of more than 1500 enzyme variants per experiment. For 1036 mutants of the alkaline phosphatase...

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Identifiers

PMID: 34437092
DOI: 10.1126/science.abf8761
PMCID: PMC8454890

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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