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Article

Mapping and Cloning of Chemical Induced Mutations by Whole-Genome Sequencing of Bulked Segregants.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017

Hua Jian, Wang Shuai, Sun Qi

Abstract excerpt

Here we describe a method of gene mapping and cloning of chemical induced mutations using next-generation sequencing on their backcrossed segregants. This method utilizes polymorphisms induced by chemical mutagens for mapping and therefore does not require outcrossing to a different background or a large segregating population. It can be used to clone causal mutations generated in various accessions or mutant...

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PMID: 28220434
DOI: 10.1007/978-1-4939-6859-6_24

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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