Article
Rapid recombination mapping for high-throughput genetic screens in Drosophila.
G3 (Bethesda, Md.) - 9 Dec 2013
Sapiro Anne L, Ihry Robert J, Buhr Derek L, Konieczko Kevin M, Ives Sarah M, Engstrom Anna K, Wleklinski Nicholas P, Kopish Kristin J, Bashirullah Arash
Abstract excerpt
Mutagenesis screens are a staple of classical genetics. Chemical-induced mutations, however, are often difficult and time-consuming to identify. Here, we report that recombination analysis with pairs of dominant visible markers provides a rapid and reliable strategy to map mutations in Drosophila melanogaster. This method requires only two generations and a total of six crosses in vials to estimate the genetic...
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