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Identification of EMS-induced causal mutations in Arabidopsis thaliana by next-generation sequencing.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014

Uchida Naoyuki, Sakamoto Tomoaki, Tasaka Masao, Kurata Tetsuya

Abstract excerpt

Emerging next-generation sequencing (NGS) technologies are powerful tools for the identification of causal mutations underlying phenotypes of interest in Arabidopsis thaliana. Based on a methodology termed bulked segregant analysis (BSA), whole-genome sequencing data are derived from pooled F2 segregants after crossing a mutant to a different polymorphic accession and are analyzed for single nucleotide...

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PMID: 24057371
DOI: 10.1007/978-1-62703-580-4_14

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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