Identification of EMS-induced causal mutations in a non-reference Arabidopsis thaliana accession by whole genome sequencing.

doi:10.1093/pcp/pcr029

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Article

Identification of EMS-induced causal mutations in a non-reference Arabidopsis thaliana accession by whole genome sequencing.

Plant & cell physiology - 1 Apr 2011

Uchida Naoyuki, Sakamoto Tomoaki, Kurata Tetsuya, Tasaka Masao

Abstract excerpt

The most frequently used method to identify mutations induced by a commonly used mutagen, EMS (ethyl methane sulfonate), in Arabidopsis thaliana has been map-based cloning. The first step of this method is crossing a mutant with a plant of another accession as it requires polymorphisms between accessions for linkage analysis. Therefore, to perform the method routinely, it is greatly preferred to use accession...

Topics

Arabidopsis
Arabidopsis Proteins
Carrier Proteins
Chromosome Mapping
Crosses, Genetic
DNA Mutational Analysis
DNA, Plant
Ethyl Methanesulfonate
Genetic Linkage
Genetic Loci
Genome, Plant

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Genome-Wide Association Study

High-Throughput Nucleotide Sequencing

Homozygote

Mutagens

Mutation

Phenotype

Polymorphism, Single Nucleotide

Source

Open in PubMed

Identifiers

PMID: 21398646
DOI: 10.1093/pcp/pcr029

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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