Article
Identification of EMS-induced causal mutations in a non-reference Arabidopsis thaliana accession by whole genome sequencing.
Plant & cell physiology - 1 Apr 2011
Uchida Naoyuki, Sakamoto Tomoaki, Kurata Tetsuya, Tasaka Masao
Abstract excerpt
The most frequently used method to identify mutations induced by a commonly used mutagen, EMS (ethyl methane sulfonate), in Arabidopsis thaliana has been map-based cloning. The first step of this method is crossing a mutant with a plant of another accession as it requires polymorphisms between accessions for linkage analysis. Therefore, to perform the method routinely, it is greatly preferred to use accession...
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