Article
Identification of an EMS-induced causal mutation in a gene required for boron-mediated root development by low-coverage genome re-sequencing in Arabidopsis.
Plant signaling & behavior - 1 Jan 2013
Tabata Ryo, Kamiya Takehiro, Shigenobu Shuji, Yamaguchi Katsushi, Yamada Masashi, Hasebe Mitsuyasu, Fujiwara Toru, Sawa Shinichiro
Abstract excerpt
Next-generation sequencing (NGS) technologies enable the rapid production of an enormous quantity of sequence data. These powerful new technologies allow the identification of mutations by whole-genome sequencing. However, most reported NGS-based mapping methods, which are based on bulked segregant analysis, are costly and laborious. To address these limitations, we designed a versatile NGS-based mapping method...
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