Article
The use of BRAF V600E mutation-specific immunohistochemistry in pediatric Langerhans cell histiocytosis.
Hematological oncology - 1 Feb 2018
Ballester Leomar Y, Cantu Miguel D, Lim Karen P H, Sarabia Stephen F, Ferguson Lizmery Suarez, Renee Webb C, Allen Carl E, McClain Kenneth L, Mohila Carrie A, Punia Jyotinder N, Roy Angshumoy, López-Terrada Dolores H, John Hicks M, Fisher Kevin E
Abstract excerpt
BRAF p.V600E mutations are detected in greater than 50% of pediatric Langerhans cell histiocytosis (LCH) lesions. However, the use of mutation-specific BRAF V600E immunohistochemistry (IHC) as a surrogate for molecular testing in pediatric LCH is unknown. We tested the mutation-specific BRAF V600E monoclonal antibody (clone VE1) in formalin-fixed, paraffin-embedded LCH samples from 26 pediatric patients (14 males...
Topics
- Adolescent
- Child
- Child, Preschool
- Female
- Histiocytosis, Langerhans-Cell
- Humans
- Immunohistochemistry
- Infant
- Male
- Mutation