Article
H3F3A mutation in giant cell tumour of the bone is detected by immunohistochemistry using a monoclonal antibody against the G34W mutated site of the histone H3.3 variant.
Histopathology - 1 Jul 2017
Lüke Julian, von Baer Alexandra, Schreiber Jordan, Lübbehüsen Christoph, Breining Thomas, Mellert Kevin, Marienfeld Ralf, Schultheiss Markus, Möller Peter, Barth Thomas F E
Abstract excerpt
AIMS: Giant cell tumour of the bone (GCTB) is a neoplasm predominantly of long bones characterized by the H3F3A mutation G34W. Conventional diagnosis is challenged by the tumour's giant cell-rich morphology, which overlaps with other giant cell-containing lesions of the bone. Recently, a monoclonal antibody specific for the H3F3A mutation has been generated. Our aim was to test this antibody on a cohort of giant...
Topics
- Adolescent
- Adult
- Aged, 80 and over
- Antibodies, Monoclonal
- Bone Neoplasms
- DNA Mutational Analysis
- Female
- Giant Cell Tumor of Bone
- Histones
- Humans
- Immunohistochemistry
- Male
- Mutation