Article
QMC-PCRx: a novel method for rapid mutation detection.
Journal of clinical pathology - 1 Aug 2017
Ebili Henry O, Hassall James, Asiri Abutaleb, Ham-Karim Hersh, Fadhil Wakkas, Agboola Ayodeji Johnson, Ilyas Mohammad
Abstract excerpt
AIMS: We previously described the quick multiplex consensus PCR (QMC-PCR) as a method for rapid mutation screening in low-quality template. QMC-PCR has two-stages: a prediagnostic multiplex (PDM) reaction followed by a single specific diagnostic reaction with high-resolution melting (HRM) analysis. We aimed to develop QMC-PCRx in which second stage was multiplexed to allow testing of multiple targets. METHODS:...
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