Article
Directed Evolution of Protein Catalysts.
Annual review of biochemistry - 20 Jun 2018
Zeymer Cathleen, Hilvert Donald
Abstract excerpt
Directed evolution is a powerful technique for generating tailor-made enzymes for a wide range of biocatalytic applications. Following the principles of natural evolution, iterative cycles of mutagenesis and screening or selection are applied to modify protein properties, enhance catalytic activities, or develop completely new protein catalysts for non-natural chemical transformations. This review briefly surveys...
Topics
- Animals
- Biocatalysis
- Directed Molecular Evolution
- Drug Design
- Enzymes
- Genetic Variation
- High-Throughput Screening Assays
- Humans
- Metabolic Networks and Pathways
- Models, Molecular
- Protein Engineering
- Proteins
- Selection, Genetic
- Stereoisomerism
- Substrate Specificity