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Article

Multiple Site-Directed and Saturation Mutagenesis by the Patch Cloning Method.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017

Taniguchi Naohiro, Murakami Hiroshi

Abstract excerpt

Constructing protein-coding genes with desired mutations is a basic step for protein engineering. Herein, we describe a multiple site-directed and saturation mutagenesis method, termed MUPAC. This method has been used to introduce multiple site-directed mutations in the green fluorescent protein gene and in the moloney murine leukemia virus reverse transcriptase gene. Moreover, this method was also successfully...

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PMID: 27709586
DOI: 10.1007/978-1-4939-6472-7_22

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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