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Article

Directed evolution of P450cin for mediated electron transfer.

Protein engineering, design & selection : PEDS - 1 Feb 2017

Belsare Ketaki D, Horn Thomas, Ruff Anna Joëlle, Martinez Ronny, Magnusson Anders, Holtmann Dirk, Schrader Jens, Schwaneberg Ulrich

Abstract excerpt

Directed evolution is a powerful method to optimize enzyme properties for application demands. Interesting targets are P450 monooxygenases which catalyze the stereo- and regiospecific hydroxylation of chemically inert C-H bonds. Synthesis employing P450s under cell-free reaction conditions is limited by low total turnover numbers, enzyme instability, low product yields and the requirement of the expensive...

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PMID: 28007937
DOI: 10.1093/protein/gzw072

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