Article
Understanding the Directed Evolution of a Natural-like Efficient Artificial Metalloenzyme.
The journal of physical chemistry. B - 12 Dec 2024
Mukherjee Anagh, Roy Subhendu
Abstract excerpt
The artificial metalloenzyme containing iridium in place of iron along with four directed evolution mutations C317G, T213G, L69V, and V254L in a natural cytochrome P450 presents an important milestone in merging the extraordinary efficiency of biocatalysts with the versatility of small molecule chemical catalysts in catalyzing a new-to-nature carbene insertion reaction. This is a show-stopper enzyme, as it...
Topics
- Molecular Dynamics Simulation
- Directed Molecular Evolution
- Cytochrome P-450 Enzyme System
- Iridium
- Quantum Theory
- Metalloproteins
- Biocatalysis
- Catalytic Domain
- Mutation
- Methane
- Protein Conformation