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Article

An Evolved Orthogonal Enzyme/Cofactor Pair.

Journal of the American Chemical Society - 28 Sept 2016

Reynolds Evan W, McHenry Matthew W, Cannac Fabien, Gober Joshua G, Snow Christopher D, Brustad Eric M

Abstract excerpt

We introduce a strategy that expands the functionality of hemoproteins through orthogonal enzyme/heme pairs. By exploiting the ability of a natural heme transport protein, ChuA, to promiscuously import heme derivatives, we have evolved a cytochrome P450 (P450BM3) that selectively incorporates a nonproteinogenic cofactor, iron deuteroporphyrin IX (Fe-DPIX), even in the presence of endogenous heme. Crystal...

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Identifiers

PMID: 27575374
DOI: 10.1021/jacs.6b05847

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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