High-Throughput Screening of Co-Protoporphyrin IX-Binding Proteins for Enhanced Hydrogen Production.

Abstract excerpt

Artificial metalloenzymes incorporating cobalt protoporphyrin IX (Co-PPIX) are promising for sustainable hydrogen production; however, slow protein preparation and a lack of suitable detection methods limit the systematic optimization of their catalytic performance. Here, we report a streamlined workflow that combines the direct in vivo incorporation of Co-PPIX into cytochrome b562 (cyt b562) variants with a...

Topics

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• Catalysis
• Cobalt
• Cytochrome b Group
• High-Throughput Screening Assays
• Hydrogen