Article
Biocatalytic conversion of avermectin to 4''-oxo-avermectin: improvement of cytochrome p450 monooxygenase specificity by directed evolution.
Applied and environmental microbiology - 1 Jul 2007
Trefzer Axel, Jungmann Volker, Molnár István, Botejue Ajit, Buckel Dagmar, Frey Gerhard, Hill D Steven, Jörg Mario, Ligon James M, Mason Dylan, Moore David, Pachlatko J Paul, Richardson Toby H, Spangenberg Petra, Wall Mark A, Zirkle Ross, Stege Justin T
Abstract excerpt
Discovery of the CYP107Z subfamily of cytochrome P450 oxidases (CYPs) led to an alternative biocatalytic synthesis of 4''-oxo-avermectin, a key intermediate for the commercial production of the semisynthetic insecticide emamectin. However, under industrial process conditions, these wild-type CYPs showed lower yields due to side product formation. Molecular evolution employing GeneReassembly was used to improve...
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