Article
Laboratory evolution of P450 BM3 for mediated electron transfer yielding an activity-improved and reductase-independent variant.
Protein engineering, design & selection : PEDS - 1 Jan 2008
Nazor Jovana, Dannenmann Stephanie, Adjei Richard Obeng, Fordjour Yaw Boafo, Ghampson Isaac Tyrone, Blanusa Milan, Roccatano Danilo, Schwaneberg Ulrich
Abstract excerpt
One of the main obstacles in employing P450 monooxygenases for preparative chemical syntheses in cell-free systems is their requirement for cofactors such as NAD(P)H. In order to engineer P450 BM3 from Bacillus megaterium for cost-effective process conditions in vitro, a validated medium throughput screening system based on cheap Zn dust as an electron source and Cobalt(III)sepulchrate (Co(III)sep) as a mediator...
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