Article
A serine-substituted P450 catalyzes highly efficient carbene transfer to olefins in vivo.
Nature chemical biology - 1 Aug 2013
Coelho Pedro S, Wang Z Jane, Ener Maraia E, Baril Stefanie A, Kannan Arvind, Arnold Frances H, Brustad Eric M
Abstract excerpt
Whole-cell catalysts for non-natural chemical reactions will open new routes to sustainable production of chemicals. We designed a cytochrome 'P411' with unique serine-heme ligation that catalyzes efficient and selective olefin cyclopropanation in intact Escherichia coli cells. The mutation C400S in cytochrome P450(BM3) gives a signature ferrous CO Soret peak at 411 nm, abolishes monooxygenation activity, raises...
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