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Article

Insightful directed evolution of Escherichia coli quorum sensing promoter region of the lsrACDBFG operon: a tool for synthetic biology systems and protein expression.

Nucleic acids research - 1 Dec 2016

Hauk Pricila, Stephens Kristina, Mckay Ryan, Virgile Chelsea Ryan, Ueda Hana, Ostermeier Marc, Ryu Kyoung-Seok, Sintim Herman O, Bentley William E

Abstract excerpt

Quorum sensing (QS) regulates many natural phenotypes (e.q. virulence, biofilm formation, antibiotic resistance), and its components, when incorporated into synthetic genetic circuits, enable user-directed phenotypes. We created a library of Escherichia coli lsr operon promoters using error-prone PCR (ePCR) and selected for promoters that provided E. coli with higher tetracycline resistance over the native...

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Share to Escherichia coli Proteins
Share to Gene Expression Regulation, Bacterial

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 27915294
DOI: 10.1093/nar/gkw981
PMCID: PMC5137460

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CRP

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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