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Directed evolution of RhlI to generate new and increased quorum sensing signal molecule catalytic activities.

Enzyme and microbial technology - 1 Mar 2020

Zhang Biao, Ren Lili, Xu Dayong, Wang Haonan, Chen Zhuo, Zhang Bingrong, Zeng Xin, Sun Lianhong, Li Feng

Abstract excerpt

Quorum sensing is a population density-dependent gene expression regulation mechanism in bacteria. The substrate specificity of RhlI, an enzyme in the RhlI-RhlR quorum sensing system of Pseudomonas aeruginosa, was explored by directed evolution to gain insight into the molecular mechanisms of quorum sensing. RhlI catalyzes S-adenosyl methionine and butanoyl or hexanoyl acyl carrier protein to form N-butanoyl...

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PMID: 32044022
DOI: 10.1016/j.enzmictec.2019.109475

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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