Article
Improved DOP-PCR (iDOP-PCR): A robust and simple WGA method for efficient amplification of low copy number genomic DNA.
PloS one - 1 Jan 2017
Blagodatskikh Konstantin A, Kramarov Vladimir M, Barsova Ekaterina V, Garkovenko Alexey V, Shcherbo Dmitriy S, Shelenkov Andrew A, Ustinova Vera V, Tokarenko Maria R, Baker Simon C, Kramarova Tatiana V, Ignatov Konstantin B
Abstract excerpt
Whole-genome amplification (WGA) techniques are used for non-specific amplification of low-copy number DNA, and especially for single-cell genome and transcriptome amplification. There are a number of WGA methods that have been developed over the years. One example is degenerate oligonucleotide-primed PCR (DOP-PCR), which is a very simple, fast and inexpensive WGA technique. Although DOP-PCR has been regarded as...
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